The ISSR primers were made by Sangon Inc. DNA samples were stored at -20 ℃ until use. Total genomic DNA was extracted from vivisectional foot muscle of two-year-old Solen strictus with standard method.
The objectives of present study are: 1) use clear amplified ISSR fragments to examine the genetic variation within and among populations of Solen strictus 2) lay a foundation of selecting the parent Solen strictus for artificial propagation. The samples of the five populations were taken from the off-shores of Dalian (DL), Yantai (YT), Laizhou (LZ), Qingdao (QD) and Ganyu (GY), respectively.
In this study, genetic diversity in five different geographical populations of the Solen strictus were analyzed by the Inter-Simple Sequence Repeat (ISSR) markers. The Solen strictus distributes widely along the coasts of the Bohai Sea and the Yellow Sea in China, where is a commercial important and potential mariculture species.
Abstract： The razor shell Solen strictus is a member of family Solenidae (Veneroida) bivalve.
